Transcriptomic Profiling of Adipose Tissue in Obese Women in Response to Acupuncture Catgut Embedding Therapy with Moxibustion



Transcriptomic Profiling of Adipose Tissue in Obese Women in Response to Acupuncture Catgut Embedding Therapy with Moxibustion


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Objective: Complementary and alternative medicine, such as Traditional Chinese Medicine, represents an efficient therapeutic option for obesity control. It was previously reported that acupuncture catgut embedding therapy (ACET) with moxibustion reduces body weight and reverts insulin resistance in obese women. This study aimed to evidence changes in adipokines and gene expression in adipose tissue that could explain the effects of ACET with moxibustion. Design: Overweight/obese women were treated with ACET with moxibustion or sham acupuncture as control. Peripheral blood samples and fat biopsies were taken before and after intervention. Circulating adipokines (leptin, adiponectin, tumor necrosis factor alpha, and resistin) were quantified by enzyme-linked immunosorbent assay. Gene expression in adipose tissue was determined by cDNA microarray assays and assessed by quantitative reverse transcription real-time polymerase chain reaction. Results: ACET with moxibustion did not modify circulating adipokines levels. However, correlations with anthropometric and biochemical parameters were affected. Interestingly, transcriptional changes in adipose tissue revealed the modulation of genes participating in homeostasis control, lipid metabolism, olfactory transduction, and gamma-aminobutyric acid signaling pathway. Conclusions: The effects of ACET with moxibustion on body weight and insulin resistance were associated with the regulation of biochemical events that are altered in obesity.


Accession Number: 117380212; Source Information: Aug2016, Vol. 22 Issue 8, p658; Subject Term: INSULIN resistance -- Prevention; Subject Term: LIPID metabolism; Subject Term: OBESITY -- Treatment; Subject Term: ANALYSIS of DNA; Subject Term: ACUPUNCTURE; Subject Term: ADIPOSE tissues; Subject Term: BIOPSY; Subject Term: BLOOD analysis; Subject Term: CORRELATION (Statistics); Subject Term: ENZYME-linked immunosorbent assay; Subject Term: GENE expression; Subject Term: GENES; Subject Term: HOMEOSTASIS; Subject Term: MOXIBUSTION; Subject Term: POLYMERASE chain reaction; Subject Term: RESEARCH -- Finance; Subject Term: T-test (Statistics); Subject Term: TUMOR necrosis factor; Subject Term: WEIGHT loss; Subject Term: RANDOMIZED controlled trials; Subject Term: MICROARRAY technology; Subject Term: ADIPONECTIN; Subject Term: ; Number of Pages: 11p; ; Document Type: Article;

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