||Objective: To observe the effect of acupuncture at Shenmai (13L 62) and Zhaohai (K1 6) on polysomnography (PSG) in primary insomnia. Methods: 120 patients were randomly divided into 3 groups, with group A receiving acupuncture at Shenmai (BL 62) with reducing method, group B receiving acupuncture at Zhaohai (KI 6) with enhancing method, and group C receiving acupuncture at Shenmai (BL 62) and Zhaohai (KI 6) with reducing and enhancing method, respectively. Forty healthy volunteers were enrolled as the control group. PSG examination was given to patients in both groups before and after treatment. Self-controlled study was conducted in the treatment group, and the change of indicators in the three treatment groups and between all four groups were compared and observed. Results: Significant difference was observed in indicators before and after treatment in both group A and B (P < 0.05), but influence on sleep cycle and sleep structure was different. All indicators before and after treatment in group C showed significant difference (P < 0.05 or P < 0.01). Comparing with group A, B and C, a significant difference was observed in the indicators (P< 0.05 or P‹ 0.01) after treatment compared with control group. Group C showed no significant difference (P> 0.05) compared with control group after treatment. Conclusion: the acupuncture combination of Zhaohai (KI 6) and Shenmai (BL 62) could obviously improve the sleep quality.