||Objective: To observe the effects on exercise-induced fatigue with scalp penetration acupuncture. Methods: 60 athletes who train in 800-meter running were divided into 3 random groups. The Treatment Group was treated with head acupuncture and body acupuncture, while the Control Group was treated with body acupuncture. The Model Group received no treatment. The use of preventive acupuncture was applied 1 hour before training. The athletes' heart rate was recorded along with the hydracrylic acid, urea nitrogen, creatine kinase, and cruarin at the first and last day of training. Results: Compared with the Model Group, the Treatment Group had a rise in Hb levels and a decrease in heart rate (P< 0.05), along with a markedly decrease in LA, BUN and CK (P<0.01). Compared with the Control Group, treatment can increase Hb and decrease heart rate (P< 0.05), and decrease LA, BUN and CK (P< 0.05). Comparison among the 3 groups (P> 0.05) had no significant difference. Conclusion: Scalp penetration acupuncture can improve athletes' internal environment and increase the ability of contra-fatigue.