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Author (up) Ning Wang; Sheng-Feng Lu; Hui Chen; Jian-Fei Wang; Shu-Ping Fu; Chen-Jun Hu; Yi Yang; Fan-Rong Liang; Bing-Mei Zhu url  doi
  Title A protocol of histone modification-based mechanistic study of acupuncture in patients with stable angina pectoris Type of Study Journal Article
  Year 2015 Publication BMC Complementary & Alternative Medicine Abbreviated Journal Bmc Complement Altern Med  
  Volume 15 Issue 1 Pages 1-11  
  Keywords Electroacupuncture; Angina, Stable -- Therapy; Proteins -- Metabolism; Protocols; Human; Randomized Controlled Trials; Double-Blind Studies; Random Assignment; Patient Selection; Adult; Middle Age; Aged; Aged, 80 and Over; Male; Female; Polymerase Chain Reaction; Descriptive Statistics; Kruskal-Wallis Test; Chi Square Test; Paired T-Tests; Wilcoxon Signed Rank Test; Confidence Intervals; Scales; Fisher's Exact Test; Correlation Coefficient; China; Funding Source  
  Abstract Background: Angina pectoris (Angina) is a medical condition related to myocardial ischemia. Although acupuncture has been widely accepted as a clinical approach for angina, there is no sufficient evidence of its effectiveness against this syndrome, and its mechanisms have not yet been well elucidated. We develop this protocol to confirm the clinical efficacy of electro-acupuncture on stable angina pectoris by needling on acupoint Neiguan (PC6). Furthermore, we employ high-throughput sequencing technology to investigate the gene expression profiling and determine involvement of histone modifications in the regulation of genes after electro-acupuncture treatment. Methods/Design: A randomized, controlled, double-blinded (assessor and patients) trial will be carried out. Sixty participants will be randomly assigned to two acupuncture treatment groups and one control group in a 1:1:1 ratio. Participants in acupuncture groups will receive 12 sessions of electro-acupuncture treatment across 4 weeks, followed by a 12-week randomization period. The acupuncture groups are divided into Neiguan (PC6) on Pericardium Meridian of Hand-jueyin or a non-acupoint. The primary clinical measure of effect is the frequency of angina attacks between these groups for four weeks after randomization. RNAs are extracted from peripheral neutrophils collected from all participants on day 0, day 30, and week 16, and are processed to RNA-Seq. We then investigate profiles of histone modifications by ChIP-Seq, for H3 Lysine 4 (H3K4me) and acetylation of H3 Lysine 27 (H3K27ac), in the presence or absence of acupuncture treatment. Discussion: This study determines the efficacy and mechanisms of electro-acupuncture on stable angina pectoris. We focus on effectiveness of acupuncture on alleviating symptoms of myocardial ischemia and the gene regulation and the chromatin remodeling marks, including H3K4me1, H3K4me2, and H3K27ac, which could be key factors for regulating gene expressions caused by electro-acupuncture treatment at Neiguan. This is the first genome-wide study of electro-acupuncture treatment in angina patients, and will provide valuable information for future studies in the fields of acupuncture and its underlying mechanisms. Fourteen patients have been recruited since recruitment opened in November of 2012. This study is scheduled to end in November of 2014.  
  Address School of Acupuncture and Tuina, Chengdu University of Traditiona Chinese Medicine, 610075 Chengdu, Sichuan, China  
  Publisher BioMed Central
  Language Number of Treatments  
  Treatment Follow-up Frequency Number of Participants  
  Time in Treatment Condition
  Disease Category OCSI Score  
  Notes Accession Number: 109830838. Language: English. Entry Date: 20150721. Revision Date: 20150923. Publication Type: Journal Article; research; tables/charts; randomized controlled trial. Journal Subset: Alternative/Complementary Therapies; Biomedical; Europe; Expert Peer Reviewed; Peer Reviewed; UK & Ireland. Instrumentation: Self-Rating Depression Scale (SDS); Self-rating Anxiety Scale (SAS). Grant Information: The trial is sponsored and financially supported by (973 Program, No. 2012CB518501) the Ministry of Science and Technology of China.. NLM UID: 101088661. Approved no  
  Call Number OCOM @ refbase @ 109830838 Serial 2305  
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